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Characteristics of mesenchymal stem cells from various tissue source according to culture media type
Min Hee Kang1, Ji Eun Jang1, Yuyeong Yang1, Jeong Eon Lee1, and Joo youn Lee1
1, Xcell Therapeutics, 333, Yeongdong-daero, Gangnam-gu, Seoul, Republic of Korea
Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. These MSCs can be found in nearly all tissues and are mostly located in perivascular niches. MSCs have ability to secrete growth factors, differentiate into many types of cells, and can act anti-inflammatory role during inflammation and tissue injuries. These functions underlie the important physiological roles of MSC and underscore a significant potential for the clinical use of distinct populations from the various tissues. It has been also reported that culture characteristics of MSCs and the function of cultured cells may vary depending on the culture media type.
Thus, this study investigated cell biological properties, proliferative capacities, mRNA expression profile, and protein expression profile of cultured human MSCs obtained from various donor and tissue source (adipose tissue, bone marrow, umbilical cord, Wharton jelly, and iPSC) with different type of media (FBS containing media, serum free media, and serum free chemically defined media). Comparative analysis of MSCs from five different source with three types of media was performed based on proliferative capacities using multipassage assay, stem cell specific surface marker expression using FACS, differentiation potency, genetic stability using cytokinesis block micronucleus assay, senescence analysis using β-galactosidase staining, mRNA expression change using mRNA sequencing and RT-PCR, and protein expression change using LC-MS/MS and western blot.
This study provides comprehensive characteristics of MSC derived from various tissue with different culture media condition to better understand their cellular and molecular biology.